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KMID : 0613820120220040472
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Journal of Life Science
2012 Volume.22 No. 4 p.472 ~ p.477
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Isolation and Culture Medium Optimization for Thermostable Extracellular α-Amylase Production by Thermophilic Alicyclobacillus acidocaldarius
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Kumar G. Satheesh
Chandra M. Subhosh
Mallaiah K.V.
Sreenivasulu P.
Choi Yong-Lark
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Abstract
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A thermophilic Alyclobacillus.acidocaldarius, which produces thermostable α-amylase, was isolated from the hot water effluent of a boiled rice mill near Tirupati, Andhra Pradesh, India. The effect of different culture conditions on the growth and production of extracellular α-amylase by thermophilic Alyclobacillus.acidocaldarius was investigated in laboratory scale. The results showed that the optimum conditions for the production of α-amylase are a temperature of 60℃, pH of 6.0, and medium starch concentration of 1.0%, and yeast extract and tryptone of 0.2%. Surfactants, like Tween-20 and SDS, up to 0.02%, were found to increase the bacterial growth and enzymes. Further increase in their concentration resulted in significantly decreased enzyme production.
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KEYWORD
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Isolation, Alyclobacillus.acidocaldarius, α-amylase, cultural conditions, optimization
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